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icell8 cx single cell system  (TaKaRa)


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    Structured Review

    TaKaRa icell8 cx single cell system
    Icell8 Cx Single Cell System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/icell8+cx+single+cell+system/ICELL8+cx+TCR+Chip/pmc12782168-51-24-29
    Average 94 stars, based on 15 article reviews
    icell8 cx single cell system - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Transcriptomic disruption and functional hypoactivity in DYT- SGCE MGE-patterned inhibitory neurons
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase containing 10 units/mL of papain (Sigma-Aldrich) for 8 minutes at 37°C, resuspended in DPBS (Gibco), mixed with 0.5% bovine serum albumin (Sigma Aldrich) and 50 units/mL DNase I (Sigma Aldrich), and passed through a 35 μm mesh cell strainer. .. Cells were stained with ReadyProbesTM Cell Viability Imaging Kit, Blue/Red (Invitrogen) following manufacturer’s guidance, and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). .. Wells containing a single nucleus were automatically and manually selected using the ICELL8 cx CellSelect v2.5 Software (Takara).

    Article Title: Differential growth and transcriptomic profile of stem cell-derived midbrain astrocytes
    Article Snippet: Cells were dissociated with Accutase with 10 units/mL of papain (Sigma-Aldrich) for 10 minutes at 37°C and resuspended in 0.5% bovine serum albumin (Sigma Aldrich) with 50 units/mL DNase I in DPBS without calcium or magnesium (Gibco) and passed through a cell strainer with 35 μm mesh. .. Cells were stained with 1 μM SYTO16 (Invitrogen) and 0.08% (v/v) propidium iodide (part of the Invitrogen ReadyProbesTM Cell Viability Imaging Kit, Blue/Red) for 20 minutes on ice and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). .. Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic.

    Article Title: Changes in neural progenitor lineage composition during astrocytic differentiation of human iPSCs
    Article Snippet: Cells were dissociated with Accutase with 10 units/mL of papain (Sigma-Aldrich) for 10 min at 37 °C and resuspended in 0.5% bovine serum albumin (Sigma-Aldrich) with 50 units/mL DNase I in DPBS without calcium or magnesium (Gibco) and passed through a cell strainer with 35 μm mesh. .. Cells were stained with 1 μM SYTO16 (Invitrogen) and 0.08% (v/v) propidium iodide (part of the Invitrogen ReadyProbes Cell Viability Imaging Kit, Blue/Red) for 20 min on ice and then dispensed into the nano-well plates of the ICELL8 cx Single-Cell System (Takara). .. Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic.

    Article Title: Transcriptomic disruption and hypoactivity in DYT-SGCE medial ganglionic eminence-patterned inhibitory neurons.
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase (8 minutes at 37°C), resuspended in 28 DPBS, mixed with 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich), and 29 AC CE PT ED M AN US CR IP T Downloaded from https://academ ic.oup.com /brain/advance-article/doi/10.1093/brain/aw af272/8213629 by guest on 16 O ctober 2025 passed through a 35 μm mesh cell strainer. .. Cells were stained with ReadyProbesTM Cell Viability 1 Imaging Kit, Blue/Red (Invitrogen) following manufacturer’s guidance, and dispensed into the 2 nano-well plates of the ICELL8® cx Single-Cell System (Takara). ..

    Article Title: Transcriptomic disruption and hypoactivity in DYT-SGCE medial ganglionic eminence-patterned inhibitory neurons
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase (8 min at 37°C), resuspended in Dulbecco's phosphate buffered saline (DPBS), mixed with 0.5% bovine serum albumin and 50 units/ml of DNase I (Sigma Aldrich), and passed through a 35 μm mesh cell strainer. .. Cells were stained with the ReadyProbesTM Cell Viability Imaging Kit, Blue/Red (Invitrogen) following the manufacturer's guidance, and dispensed into the nano-well plates of the ICELL8 ® cx Single-Cell System (Takara). ..

    Imaging:

    Article Title: Transcriptomic disruption and functional hypoactivity in DYT- SGCE MGE-patterned inhibitory neurons
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase containing 10 units/mL of papain (Sigma-Aldrich) for 8 minutes at 37°C, resuspended in DPBS (Gibco), mixed with 0.5% bovine serum albumin (Sigma Aldrich) and 50 units/mL DNase I (Sigma Aldrich), and passed through a 35 μm mesh cell strainer. .. Cells were stained with ReadyProbesTM Cell Viability Imaging Kit, Blue/Red (Invitrogen) following manufacturer’s guidance, and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). .. Wells containing a single nucleus were automatically and manually selected using the ICELL8 cx CellSelect v2.5 Software (Takara).

    Article Title: Differential growth and transcriptomic profile of stem cell-derived midbrain astrocytes
    Article Snippet: Cells were dissociated with Accutase with 10 units/mL of papain (Sigma-Aldrich) for 10 minutes at 37°C and resuspended in 0.5% bovine serum albumin (Sigma Aldrich) with 50 units/mL DNase I in DPBS without calcium or magnesium (Gibco) and passed through a cell strainer with 35 μm mesh. .. Cells were stained with 1 μM SYTO16 (Invitrogen) and 0.08% (v/v) propidium iodide (part of the Invitrogen ReadyProbesTM Cell Viability Imaging Kit, Blue/Red) for 20 minutes on ice and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). .. Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic.

    Article Title: Changes in neural progenitor lineage composition during astrocytic differentiation of human iPSCs
    Article Snippet: Cells were dissociated with Accutase with 10 units/mL of papain (Sigma-Aldrich) for 10 min at 37 °C and resuspended in 0.5% bovine serum albumin (Sigma-Aldrich) with 50 units/mL DNase I in DPBS without calcium or magnesium (Gibco) and passed through a cell strainer with 35 μm mesh. .. Cells were stained with 1 μM SYTO16 (Invitrogen) and 0.08% (v/v) propidium iodide (part of the Invitrogen ReadyProbes Cell Viability Imaging Kit, Blue/Red) for 20 min on ice and then dispensed into the nano-well plates of the ICELL8 cx Single-Cell System (Takara). .. Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic.

    Article Title: Transcriptomic disruption and hypoactivity in DYT-SGCE medial ganglionic eminence-patterned inhibitory neurons.
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase (8 minutes at 37°C), resuspended in 28 DPBS, mixed with 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich), and 29 AC CE PT ED M AN US CR IP T Downloaded from https://academ ic.oup.com /brain/advance-article/doi/10.1093/brain/aw af272/8213629 by guest on 16 O ctober 2025 passed through a 35 μm mesh cell strainer. .. Cells were stained with ReadyProbesTM Cell Viability 1 Imaging Kit, Blue/Red (Invitrogen) following manufacturer’s guidance, and dispensed into the 2 nano-well plates of the ICELL8® cx Single-Cell System (Takara). ..

    Article Title: Transcriptomic disruption and hypoactivity in DYT-SGCE medial ganglionic eminence-patterned inhibitory neurons
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase (8 min at 37°C), resuspended in Dulbecco's phosphate buffered saline (DPBS), mixed with 0.5% bovine serum albumin and 50 units/ml of DNase I (Sigma Aldrich), and passed through a 35 μm mesh cell strainer. .. Cells were stained with the ReadyProbesTM Cell Viability Imaging Kit, Blue/Red (Invitrogen) following the manufacturer's guidance, and dispensed into the nano-well plates of the ICELL8 ® cx Single-Cell System (Takara). ..

    Single Cell:

    Article Title: Transcriptomic disruption and functional hypoactivity in DYT- SGCE MGE-patterned inhibitory neurons
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase containing 10 units/mL of papain (Sigma-Aldrich) for 8 minutes at 37°C, resuspended in DPBS (Gibco), mixed with 0.5% bovine serum albumin (Sigma Aldrich) and 50 units/mL DNase I (Sigma Aldrich), and passed through a 35 μm mesh cell strainer. .. Cells were stained with ReadyProbesTM Cell Viability Imaging Kit, Blue/Red (Invitrogen) following manufacturer’s guidance, and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). .. Wells containing a single nucleus were automatically and manually selected using the ICELL8 cx CellSelect v2.5 Software (Takara).

    Article Title: Differential growth and transcriptomic profile of stem cell-derived midbrain astrocytes
    Article Snippet: Cells were dissociated with Accutase with 10 units/mL of papain (Sigma-Aldrich) for 10 minutes at 37°C and resuspended in 0.5% bovine serum albumin (Sigma Aldrich) with 50 units/mL DNase I in DPBS without calcium or magnesium (Gibco) and passed through a cell strainer with 35 μm mesh. .. Cells were stained with 1 μM SYTO16 (Invitrogen) and 0.08% (v/v) propidium iodide (part of the Invitrogen ReadyProbesTM Cell Viability Imaging Kit, Blue/Red) for 20 minutes on ice and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). .. Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic.

    Article Title: Microbiota-derived lysophosphatidylcholine alleviates Alzheimer's disease pathology via suppressing ferroptosis.
    Article Snippet: The cell suspensions were diluted and aliquoted through 384-well plates to ICELL8 350v chips (Clontech). .. Using the ICELL8 cx Single-Cell System (Takara) and CellSelect software, the nanowells on the chip were imaged and selected manually. .. Using the ICELL8 cx Single-Cell System (Takara) and CellSelect software, the nanowells on the chip were imaged and selected manually.

    Article Title: Changes in neural progenitor lineage composition during astrocytic differentiation of human iPSCs
    Article Snippet: Cells were dissociated with Accutase with 10 units/mL of papain (Sigma-Aldrich) for 10 min at 37 °C and resuspended in 0.5% bovine serum albumin (Sigma-Aldrich) with 50 units/mL DNase I in DPBS without calcium or magnesium (Gibco) and passed through a cell strainer with 35 μm mesh. .. Cells were stained with 1 μM SYTO16 (Invitrogen) and 0.08% (v/v) propidium iodide (part of the Invitrogen ReadyProbes Cell Viability Imaging Kit, Blue/Red) for 20 min on ice and then dispensed into the nano-well plates of the ICELL8 cx Single-Cell System (Takara). .. Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic.

    Article Title: Transcriptomic disruption and hypoactivity in DYT-SGCE medial ganglionic eminence-patterned inhibitory neurons.
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase (8 minutes at 37°C), resuspended in 28 DPBS, mixed with 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich), and 29 AC CE PT ED M AN US CR IP T Downloaded from https://academ ic.oup.com /brain/advance-article/doi/10.1093/brain/aw af272/8213629 by guest on 16 O ctober 2025 passed through a 35 μm mesh cell strainer. .. Cells were stained with ReadyProbesTM Cell Viability 1 Imaging Kit, Blue/Red (Invitrogen) following manufacturer’s guidance, and dispensed into the 2 nano-well plates of the ICELL8® cx Single-Cell System (Takara). ..

    Article Title: Transcriptomic disruption and hypoactivity in DYT-SGCE medial ganglionic eminence-patterned inhibitory neurons
    Article Snippet: Day 80 neuronal cultures were dissociated with Accutase (8 min at 37°C), resuspended in Dulbecco's phosphate buffered saline (DPBS), mixed with 0.5% bovine serum albumin and 50 units/ml of DNase I (Sigma Aldrich), and passed through a 35 μm mesh cell strainer. .. Cells were stained with the ReadyProbesTM Cell Viability Imaging Kit, Blue/Red (Invitrogen) following the manufacturer's guidance, and dispensed into the nano-well plates of the ICELL8 ® cx Single-Cell System (Takara). ..

    Article Title: scTrends: A living review of commercial single-cell and spatial 'omic technologies
    Article Snippet: .. This approach is embodied by the Takara ICELL8 cx Single-Cell System, which supports the processing of 5,184 nanowells in parallel and can be leveraged to implement several different molecular protocols (e.g., SMART-seq-based single-cell RNA-seq [scRNA-seq], single-cell ATAC-seq [scATAC-seq], and single-cell TCR-seq, as well as user-designed protocols). .. This approach is embodied by the Takara ICELL8 cx Single-Cell System, which supports the processing of 5,184 nanowells in parallel and can be leveraged to implement several different molecular protocols (e.g., SMART-seq-based single-cell RNA-seq [scRNA-seq], single-cell ATAC-seq [scATAC-seq], and single-cell TCR-seq, as well as user-designed protocols).

    Software:

    Article Title: Microbiota-derived lysophosphatidylcholine alleviates Alzheimer's disease pathology via suppressing ferroptosis.
    Article Snippet: The cell suspensions were diluted and aliquoted through 384-well plates to ICELL8 350v chips (Clontech). .. Using the ICELL8 cx Single-Cell System (Takara) and CellSelect software, the nanowells on the chip were imaged and selected manually. .. Using the ICELL8 cx Single-Cell System (Takara) and CellSelect software, the nanowells on the chip were imaged and selected manually.

    RNA Sequencing:

    Article Title: scTrends: A living review of commercial single-cell and spatial 'omic technologies
    Article Snippet: .. This approach is embodied by the Takara ICELL8 cx Single-Cell System, which supports the processing of 5,184 nanowells in parallel and can be leveraged to implement several different molecular protocols (e.g., SMART-seq-based single-cell RNA-seq [scRNA-seq], single-cell ATAC-seq [scATAC-seq], and single-cell TCR-seq, as well as user-designed protocols). .. This approach is embodied by the Takara ICELL8 cx Single-Cell System, which supports the processing of 5,184 nanowells in parallel and can be leveraged to implement several different molecular protocols (e.g., SMART-seq-based single-cell RNA-seq [scRNA-seq], single-cell ATAC-seq [scATAC-seq], and single-cell TCR-seq, as well as user-designed protocols).



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